11 Vectored vaccines
Zeyu Yang, Kumar Subramaniam, and
Amine Kamen
Viral Vectors and Vaccines Bioprocessing Group,
Department of Bioengineering, McGill University, Montréal,
QC, Canada
CONTENTS
11.1
Introduction.................................................................................................269
11.2
Adenovirus-Vectored Vaccines..................................................................270
11.2.1 AdV Structure and Vector Design...............................................276
11.2.2 Production Process of AdV Vectors............................................277
11.2.2.1 Cell Line Selection ......................................................277
11.2.2.2 Limitations in the Production of AdV........................277
11.2.2.3 Productivity of AdV in Different Operation Modes ..278
11.2.2.4 Quantitation Methods of AdV Vector.........................279
11.2.3 Examples of AdV-Based Vaccines..............................................279
11.2.3.1 Veterinary Vaccines.....................................................279
11.2.3.2 AdV-Based Ebola Vaccine..........................................280
11.2.3.3 AdV-Based COVID-19 Vaccine .................................280
11.3
Vesicular Stomatitis Virus (VSV) Vectored Vaccines .............................281
11.3.1 Vesicular Stomatitis Virus ...........................................................281
11.3.2 Construction and Production Process of VSV Vectors...............281
11.3.2.1 Pseudotype and Recombinant VSV ............................281
11.3.2.2 VSV As a Vaccine Vector ..........................................283
11.3.2.3 Cell-Line Selection ......................................................283
11.3.2.4 Production Process of VSV Vector.............................285
11.3.3 Example of VSV-Based Vaccines ...............................................285
11.3.3.1 VSV-Based Ebola Vaccine..........................................285
11.3.3.2 VSV-Based COVID-19 Vaccine Candidates..............286
11.4
Overall Conclusion on Vectored Vaccines................................................286
References..............................................................................................................286
11.1
INTRODUCTION
This section will cover the design of viral vectors for vaccination and will describe in
some detail the cell-culture production and processing of adenoviral (AdV) and vesi-
cular stomatitis virus (VSV) vectored vaccines. A broad definition of recombinant viral
DOI: 10.1201/9781003229797-11
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